PHYTOCHEMICAL SCREENING OF THE METHANOLIC EXTRACT OF STEM OF TRICALYSIA SPHAEROCARPA (DALZELL EX HOOK. F,) GAMBLE

Tricalysia is a genus of the plant family Rubiaceae. Approximately 50 species distributed in subtropical and tropical regions in Asia and Africa. Some of these used as folk fore medicine as sedative, emetic, malaria/yellow fever, skin diseases and also for urine disorders. Tricalysia sphaerocarpa is commonly known as wild coffee and its synonym was Discospermum sphaerocarpum Dalzell ex Hook. F. Powdered materials were subjected to successive extraction with chloroform, diethyl ether, ethyl acetate and methanol by soxhlet method for preliminary phytochemical screening and methanol extract is used for GC-MS analysis to investigate the chemical components present in it. Totally 17 chemical compounds were identified, among which 5 belongs to fatty acid esters groups, 4 belongs to steroid groups. Of which octadecanoic acid (29.88%), n-hexadecanoic acid (15.10 %), 9, 12, 15-octadecatrienoic acid,(z,z,z)-(12.32 %) were the major constituent identified.