Epilepsy is a CNS illness in which aberrant brain activity causes seizures, as well as periods of odd behaviour, loss of awareness, and sensation loss. Excessive and aberrant neuronal activity in the cortex of the brain causes epileptic seizures. Convulsive, generalised, and partial seizures are the most frequent types of seizures. The medicinal plants are used to treat epilepsy because they have anticonvulsant action and can be tested for anticonvulsant activity using an animal model, making them a viable source for developing novel anti-epileptic chemicals. In traditional medicine, the entire plant of Limnophyton obtusifolium is used to cure epilepsy and ulcers. Our data support the concept that seizure induction lowers monoamine levels in the rat brain. Monoamines such as NA, DA, 5-HT, and GABA levels in the forebrain of Limnophyton obtusifolium extract-treated rats were dramatically recovered. In rats, MELO raises the seizure threshold and lowers vulnerability to MES and PTZ-induced seizures. As a result, we propose that a methanol extract of Limnophyton obtusifolium L. has antiepileptic activities, which might be attributable to the restoration of biogenic amines in the rat brain